Here source from Google: In Season 2, Episode 16, "The Show," Bingo and Bluey put on a play of their mother's life, and when they reach Bluey's birth, they dress Bandit up as a baby and announce, "She had a baby girl!" And a few episodes later in Season 2, Episode 25, "Double Babysitter," the girls see their Uncle Rad (Patrick Brammall), who misgenders Bluey but gets a quick correction from the little girl. With several instances like this, it's impossible to deny Bluey's gender identity in the series.
